Regulation of gene expression is one of the most important life processes. This regulation is essential for several target genes which ensure many processes such as cell differentiation, programmed cell death or cell stress response, depend on it.
Morpholino oligos interference is one of the advanced tools in the field of Molecular Biology, Ikan offers to knockdown expression of many target sequences in in vivo assays. The introduction of this small molecule (about 25 bp), antisense and complementary to a mRNA targets triggers to block their translation, obstructing gene expression. This study is used to search functions that are associated with many genes through a modification as well as control of normal splicing events, blocking their expression during hours.
- Validation of Knockdown model by RT-PCR or Western Blot
- Monitorization of the development process