The COLON-HDAC6 project begins to have its first clarifying results.
An MTS test has been carried out with the objective of evaluating the cell proliferation, viability and cytotoxicity.
In the following figure, an MTS is shown for the 4 colonic stable cell lines with different concentrations, both with and without medium change. It is observed how the viability of the cells at concentrations of 200 uM for UDCA-1 decreases in the four cases.
An absorbance assay has also been performed for the compounds UDCA-1 and UDCA-8. The influence of the precipitates formed with high concentrations can be verified, 400uM in the case of UDCA-1 and 200uM and 400uM in the case of UDCA-8.
To evaluate the concentrations reached as efficient for UDCA-1 in vitro, an avatar model with primary tumor formation has been created from the stable cell line HCT116 CRC. Since the toxicity test performed for the UDCA drugs showed 100% survival at 100 uM, we increased the concentration to 200 uM in this experiment, noting that the injected fish were not affected by that increase in concentration.
The results indicate that UDCA-1 in vitro has an effect on the 4 stable CRC cell lines and it is possible to work with this compound without having precipitates in the medium up to 200uM, it is also the one that dissolves best when using beta cyclodextrin. On the other hand, UDCA-8 is also promising, and its effect will be studied in the next phase of the project.